In vitro investigation of the antimicrobial activity of Dezfuli orange peel essential oil with and without common antibiotics on some pathogenic bacteria

Authors
Mohammad Noshad 1
Behrooz Alizadeh behbahani 1
Hossein Jooyandeh 2
Mostafa Rahmati-Joneidabad 3
Ebrahimi ابرهیمی همتی کیخا 4
Mitra Ghodsi Sheikhjan 5
1 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 3- Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
4 4- MSc. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
5 5- DVM, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
10.52547/fsct.18.111.159
Abstract
Given the rise in the infectious diseases caused by pathogens, identification of medicinal plants and purification of their nutraceuticals can be useful in treating such diseases. In this experimental study, the antimicrobial activity of Dezfuli orange peel essential oil was examined on 3 Gram-negative strains (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi) and 5 Gram-positive strains (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Staphylococcus epidermidis and Listeria innocua) through agar disc diffusion, agar well diffusion, minimum inhibitory concentration (microdilution broth) and minimum bactericidal concentration. Furthermore, the interactions between the essential oil and chloramphenicol, gentamycin and tetracycline were investigated. The results of disc diffusion showed that the longest and shortest diameters of the growth inhibition zone belonged to B. cereus (21.20 mm) and L. innocua (13.20 mm) respectively. In the agar diffusion test, S. aureus and E. coli respectively had the longest (17.30 mm) and shortest (11.10 mm) diameters of the inhibition zone. The minimum inhibitory concentration was equal to 25, 400, 50, 12.5, 25, 25, 400 and 12.5 mg/ml for E. coli, P. aeruginosa, S. typhi, S. aureus, B. cereus, B. subtilis, S. epidermidis and L. innocua respectively. The minimum bactericidal concentration of the essential oil was greater than 400 mg/ ml for all the strains.
Keywords
Orange peel essential oil
Antimicrobial activity
natural preservative
Common antibiotics

Subjects

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