تولید باکتریوسین در فرآیند غیر مداوم تخمیر ضایعات لبنی توسط لاکتوباسیلوس اسیدوفیلوس LA5 و بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB12

نویسندگان
صابر امیری 1
رضا رضایی مکرم 2
محمود صوتی خیابانی 3
محمود رضازاد باری 4
محمد علیزاده 4
1 دکترای تخصصی میکروبیولوژی مواد غذایی، گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه تبریز، تبریز
2 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه تبریز، تبریز،
3 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه تبریز، تبریز
4 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه
چکیده
هدف پژوهش حاضر بررسی عوامل موثر بر فرآیند تخمیر در تولید باکتریوسین توسط دو باکتری پروبیوتیک تجاری با استفاده از ضایعات کارخانجات لبنی به عنوان محیط کشت می­باشد. از این رو اثر متغیرهای مستقل شامل: دمای گرمخانه گذاری (30، 34 و 38 درجه سانتی­گراد)، pH اولیه (5، 6 و 7)، مدت زمان گرمخانه گذاری (12، 30 و 48 ساعت)، غلظت عصاره مخمر (صفر، 2 و 4 درصد)، نوع باکتری پروبیوتیک (لاکتوباسیلوس اسیدوفیلوس LA5 و بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB12) و نوع محیط کشت (آب پنیر و پرمیات شیر) استفاده از طرح پایه کاملاً تصادفی به صورت آرایش فاکتوریل دو سطحی، بررسی شد. نتایج نشان داد که دمای و زمان گرمخانه گذازی و همچنین نوع محیط کشت بر میزان باکتریوسین تولید اثر معنی دار داشت (05/0p<). همچنین دما، غلظت عصاره مخمر، نوع محیط کشت و نوع کشت باکتری­ها اثر معنی داری بر میزان زیست توده داشت (05/0p<). pH اولیه و نوع محیط کشت بر مقدار پروتئین کل اثر معنی دار داشت (05/0p<). بر اساس نتایج دمای گرمخانه گذاری، مدت زمان گرمخانه گذاری، غلظت عصاره مخمر، نوع محیط کشت و نوع باکتری پروبیوتیک بر اسیدیته قابل تیتراسیون تأثیر معنی دار داشتند (05/0p<). مقادیر فعالیت باکتریوسین، زیست توده، پروتئین کل و اسیدیته قابل تیتراسیون به ترتیب در محدوده AU/mL 1000 تا5000، g/L80/0 تا 67/8،mg/L 75/107 تا 92/351 و g/L 25/0 تا 41/1 متغیر بود. به طور کلی نتایج نشان داد که آب پنیر و باکتری لاکتوباسیلوس اسیدوفیلوس LA5 به ترتیب محیط کشت و باکتری مناسب جهت تولید باکتریوسین بودند.
کلیدواژه‌ها
باکتریوسین
ضایعات لبنی
لاکتوباسیلوس اسیدوفیلوس LA5
بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB12
تخمیر غیرمداوم

موضوعات

عنوان مقاله English

Production of bacteriocin in batch fermentation of dairy effluents by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12

نویسندگان English

Saber Amiri 1
Reza Rezayi mokarram 2
Mahmoud Sowti Khiabani 3
Mahmoud Rezazadeh Bari 4
Mohammad Alizadeh 4
1 PhD, Food Microbiology, Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده English

The purpose of this study was to investigate the factors affecting the fermentation process in the production of bacteriosin by two commercial probiotic bacteria in dairy factories effluents as a culture medium. Therefore, the effect of independent variables, including incubation temperature (30, 34 and 38 °C), initial pH (5, 6 and 7), duration of incubation (12, 30 and 48 hours), yeast extract concentration (0, 2 and 4 %), probiotic bacterial species (L. acidophilus LA5 and B. animalis subsp. lactis BB12) and culture medium (cheese whey and milk permeate) was studied using a completely randomized design with two-level factorial arrangement. The results showed that the temperature and time of incubation as well as the culture medium had a significant effect on bacteriosin production (p<0.05). Also, temperature, yeast extract concentration, culture medium type and culture of bacteria had a significant effect on biomass (p<0.05). Initial pH and culture medium had a significant effect on total protein content (p<0.05). Based on the results of incubation temperature, duration of incubation, yeast extract concentration, type of culture medium and type of probiotic bacteria had a significant effect on the titratable acidity (p<0.05). The levels of bacteriosin activity, biomass, total protein, and titratable acidity were in the range of 1000 to 5000 AU/mL, 0.80 to 8.67 g/L, 107.75 to 351.92 mg/L and 0.25 to 1.41 g/L, respectively. In general, the results showed that cheese whey and L. acidophilus LA5 is the suitable culture medium and bacterium for producing bacteriosin, respectively.

کلیدواژه‌ها English

Bacteriocin
dairy effluents
Lactobacillus acidophilus LA5
Bifidobacterium animalis subsp. lactis BB12
Batch fermentation
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