Investigation of PhysicoMechanical Properties of Functional Gummy Candy Fortified with Encapsulated Fish Oil in Chitosan-Stearic Acid Nanogel by Pickering Emulsion Method

Author
Assistant Prof. in Faculty of Agriculture, Shahrood University of Technology
Abstract
However, the beneficial effects of fish oil, which is fortified in omega-3 fatty acids, is known. But its high sensitivity to oxidation and the formation of undesirable compounds has limited its use in food systems. Therefore, the purpose of this study was the encapsulation of fish oil in chitosan-stearic acid nanogel, by Pickering emulsion method and import it into a food system (Gummy candy) and investigation the physicomechanical properties of the product. Initially, chitosan-stearic acid nanogel was created by self-assemble method and the results of FTIR confirmed the successful connection between chitosan and stearic acid. Also, SEM image showed that the nanoparticles formation was spherical nearly. In the next step, fish oil Pickering emulsions were prepared in different concentrations using chitosan-stearic acid nanogel and used in formulation of gummy candy and in continue, the texture profile analysis (TPA) and also the measurements of the color indexes of the samples were performed. The results of TPA showed that the existence of the nanogel, increased the Springiness (from% 88.5 to% 92.5) and adhesion (from Ns 0.33 to Ns 0.63) and reduced the hardness (from N 178.6 to N 125.8) of texture of gummy candy samples. On the other hand, the presence of fish oil reduced all of the texture indexes. About the color indexs, the nanogel decreased the L (from 58.13 to 56.46) and a (from -5.5 to -4.4) indexes. Also, the results showed that fish oil in combination with nanogels reduced the color indexes.
Keywords

Subjects


[1] Kaushik, P., Dowling, K., Barrow, C. J., & Adhikari, B. 2015. Microencapsulation of omega-3 fatty acids: A review of microencapsulation and characterization methods. Journal of functional foods, 19, 868-881.
[2] Aveyard, R., Binks, B. P., & Clint, J. H. 2003. “Emulsions stabilised solely by colloidal particles”. Advances in Colloid and Interface Science, 100, 503-546.
[3] Khalili, S. T., Mohsenifar, A., Beyki, M., Zhaveh, S., Rahmani-Cherati, T., Abdollahi, A., & Tabatabaei, M. 2015. Encapsulation of Thyme essential oils in chitosan-benzoic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. LWT-Food Science and Technology, 60(1), 502-508.
[4] Elsabee, M. Z., Morsi, R. E., & Al-Sabagh, A. M. 2009. Surface active properties of chitosan and its derivatives. Colloids and Surfaces B: Biointerfaces, 74(1), 1–16.
[5] Kargar, M., Fayazmanesh, K., Alavi, M., Spyropoulos, F., & Norton, I. T. 2012. Investigation into the potential ability of Pickering emulsions (food-grade particles) to enhance the oxidative stability of oil-in-water emulsions. Journal of Colloid and Interface Science, 366(1), 209–215.
[6] Kargar, M., Spyropoulos, F., & Norton, I. T. 2011. The effect of interfacial microstructure on the lipid oxidation stability of oil-in-water emulsions. Journal of Colloid and Interface Science, 357(2), 527–533.
[7] Atarian, M., Rajaei, A., Tabatabaei, M., Mohsenifar, A., & Bodaghi, H. 2019. Formulation of Pickering sunflower oil-in-water emulsion stabilized by chitosan-stearic acid nanogel and studying its oxidative stability. Carbohydrate polymers, 210, 47-55.
[8] Mwangi, W. W., Ho, K. W., Tey, B. T., & Chan, E. S. 2016. Effects of environmental factors on the physical stability of pickering-emulsions stabilized by chitosan particles. Food Hydrocolloids, 60, 543–550. https://doi.org/10.1016/j.foodhyd.2016.04.023
[9] Xiao, J., Wang, X., Perez Gonzalez, A. J., & Huang, Q. 2016. Kafirin nanoparticles-stabilized Pickering emulsions: Microstructure and rheological behavior. Food Hydrocolloids, 54, 30–39.
[10] Caine, W. R., Aalhus, J. L., Best, D. R., Dugan, M. E. R., & Jeremiah, L. E. 2003. Relationship of texture profile analysis and Warner-Bratzler shear force with sensory characteristics of beef rib steaks. Meat science, 64(4), 333-339.
[11] Valenzuela, C., Abugoch, L., & Tapia, C. 2013. Quinoa protein–chitosan–sunflower oil edible film: Mechanical, barrier and structural properties. LWT-Food Science and Technology, 50(2), 531-537.
[12] Benbettaïeb, N., Kurek, M., Bornaz, S., & Debeaufort, F. 2014. Barrier, structural and mechanical properties of bovine gelatin–chitosan blend films related to biopolymer interactions. Journal of the Science of Food and Agriculture, 94(12), 2409-2419.
[13] Larkin, P. 2011. Infrared and Raman spectroscopy: principles and spectral interpretation. Elsevier.
[14] Rao, K. S. V. K., Reddy, P. R., Lee, Y.-I., & Kim, C. 2012. Synthesis and characterization of chitosan--PEG--Ag nanocomposites for antimicrobial application. Carbohydrate Polymers, 87(1), 920–925.
[15] Wang, X. H., Li, D. P., Wang, W. J., Feng, Q. L., Cui, F. Z., Xu, Y. X., van der Werf, M. 2003. Crosslinked collagen/chitosan matrix for artificial livers. Biomaterials, 24(19), 3213–3220.
[16] Wang, L.-J., Hu, Y.-Q., Yin, S.-W., Yang, X.-Q., Lai, F.-R., & Wang, S.-Q. 2015. Fabrication and characterization of antioxidant pickering emulsions stabilized by zein/chitosan complex particles (ZCPs). Journal of Agricultural and Food Chemistry, 63(9), 2514–2524.
[17] Wongkongkatep, P., Manopwisedjaroen, K., Tiposoth, P., Archakunakorn, S., Pongtharangkul, T., Suphantharika, M., Wongkongkatep, J. 2012. Bacteria Interface Pickering Emulsions Stabilized by Self-assembled Bacteria–Chitosan Network. Langmuir, 28(13), 5729–5736.
[18] Pereda, M., Amica, G., & Marcovich, N. E. 2012. Development and characterization of edible chitosan/olive oil emulsion films. Carbohydrate polymers, 87(2), 1318-1325.