Comparison of the effect of physical and chemical treatments on decay control, qualitative characteristics and some flavonoids of Thomson-navel orange fruits in cold storage

Authors
1 Researcher, citrus and subtropical research center, Horticultural Research Institute, Agricultural Research, Education and Extension Organization, Ramsar, Iran
2 Professor, Department of Horticultural Science, Tarbiat Modarres University, Tehran, Iran
3 Professor, Department of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Professor, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran
Abstract
The decay of green and blue molds of Thomson navel oranges is one of the main causes of this product's decay and it can be a limiting factor in storing fruits. The aim of this study was to investigate the effects of wax, hot water, calcium chloride and commercial Tecto 60 fungicide treatments on reducing decay by inducing biosynthesis of flavonoid compounds and maintaining the postharvest quality of Thomson navel orange fruits. Fruits kept for three months in a cold storage at a temperature of 5 to 7 °C and a relative humidity of about 75 to 90%. Weight loss of fruits in waxed fruits was significantly lower than other treatments. The wounded and infected fruits did not have much chance of survival. None of the treatments were significantly effective on control of decay of non-wounded fruits. Total phenol content of fruit peel and flesh decreased during storage. Hesperidin in the peel was at the highest level in Britex wax treatment. The highest amount of scoparon of fruit peel and flesh was in hot water and calcium + wounded treatments, respectively. Although the amount of scoparon in some treatments inhibited the germination of fungal spores, it did not have the expected effect in controlling decay. The use of Tecto 60™ and hot water can significantly reduce the decay of wounded and infected fruits. The treatments were not effective in controlling the deterioration of intact fruits, which indicates the importance of preventing mechanical damage to the fruit in the pre-harvest stage.
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1. Barkai-Golan, R. 2001 Postharvest Diseases of Fruits and Vegetables: Development and Control. Amsterdam: ELSEVIER. 418.
2. Anonymous. 2017 Statistics of horticultural crops.Comunication and information technology center Tehran, IRAN: Ministry of Jihad agriculture. (in Farsi).
3. Youssef, K., Sanzani, S.M., Ligorio, A., Ippolito, A., and Terry, L.A. 2014. Sodium carbonate and bicarbonate treatments induce resistance to postharvest green mould on citrus fruit. Postharvest Biology and Technology. 87: p. 61-69.
4. Barkai-Golan, R. and Paster, N. 2008 Mycotoxins in fruits and vegetables. Academic Press.
5. Smilanick, J.L., Mansour, M.F., Gabler, F.M., and Sorenson, D. 2008. Control of citrus postharvest green mold and sour rot by potassium sorbate combined with heat and fungicides. Postharvest Biology and Technology. 47(2): p. 226-238.
6. Adaskaveg, J. 2004. Evaluation of new postharvest treatments to reduce postharvest decays and improve fruit quality in citrus packinghouse operations. Citrus Research Board. 2004 Annual Report. p. 64-65.
7. ZHU, J.-W., XIE, Q.-Y., and LI, H.-Y. 2006. Occurrence of imazalil-resistant biotype of Penicillium digitatum in China and the resistant molecular mechanism. Journal of Zhejiang University SCIENCE A. 7: p. 362-365.
8. Ortelli, D., Edder, P., and Corvi, C. 2005. Pesticide residues survey in citrus fruits. Food Additives and Contaminants. 22(5): p. 423–428.
9. Holmes, G.J. and Eckert, J.W. 1999. Sensitivity of Penicillium digitatum and P. italicum to Postharvest Citrus Fungicides in California. Phytopathology. 89: p. 716-721.
10. Vermerris, W. and Nicholson, R. 2006 Phenolic compound biochemistry. Springer Verlag.
11. Afek, U. and Sztejnberg, A. 1995. Scoparone (6,7-Dimethoxycoumarin), a Citrus Phytoalexin Involved in Resistance to Pathogens, in Handbook of Phytoalexin Metabolism and Action, M. Daniel and R.P. Purkayastha, Editors, Marcel Dekker, Inc.: New York. p. 263-286.
12. Afek, U. and Sztejnberg, A. 1988. Accumulation of scoparone, a phytoalexin associated with resistance of Citrus to Phytophthora citrophthora. Phytopathology. 78(12): p. 1678-1682.
13. Kim, J.J., Ben-Yehoshua, S., Shapiro, B., Henis, Y., and Carmeli, S. 1991. Accumulation of Scoparone in Heat-Treated Lemon Fruit Inoculated with Penicillium digitatum Sacc. Plant Physiol. 97: p. 880-885.
14. Rodov, V., Ben-Yehoshua, S., Kim, J.J., Shapiro, B., and Ittah, Y. 1992. Ultraviolet Illumination Induces Scoparone Production in Kumquat and Orange Fruit and Improves Decay Resistance. J. AMER. SOC. HORT. SCI. . 117(5): p. 788-792.
15. Ortuno, A., Botı´a, J.M., Fuster, M.D., Porras, I., Garcia-Lidon, A., and Rio, J.A.D. 1997. Effect of Scoparone (6,7-Dimethoxycoumarin) Biosynthesis on the Resistance of Tangelo Nova, Citrus paradisi, and Citrus aurantium Fruits against Phytophthora parasitica. Agric. Food Chem. 45: p. 2740-2743.
16. Afek, U., Sztejnberg, A., and Carmely, S. 1986. 6, 7-dimethoxycoumarin, a Citrus phytoalexin conferring resistance against Phytophthora gummosis. Phytochemistry. 25(8): p. 1855-1856.
17. Meyers, K.J., Watkins, C.B., Pritts, M.P., and Liu, R.H. 2003. Antioxidant and antiproliferative activities of strawberries. Journal of agricultural and food chemistry. 51(23): p. 6887-6892.
18. Fattahi, J., Hamidoghli, Y., Fotouhi, R., Ghasemnejad, M., and Bakhsi, D. 2011. Assessment of fruit quality and antioxidant activity of three citrus species during ripening. South-Western Journal of Horticulture Biology and Environment. 2(2): p. 113-128.
19. Bor, J.-Y., Chen, H.-Y., and Yen, G.-C. 2006. Evaluation of antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables. Journal of agricultural and food chemistry. 54(5): p. 1680-1686.
20. Cohen, E., Shalom, Y., and Rosenberger, I. 1990. Postharvest Ethanol Buildup and Off-flavor in Murcott 'Tangerine Fruits. Journal of the American Society for Horticultural Science. 115(5): p. 775-778.
21. Murata, T. 1997. Citrus, in Postharvest physiology and storage of tropical and subtropical fruits, S.K. Mitra, Editor CAB International,: UK. p. 21-40.
22. Ariza, M.R., Larsen, T.O., Petersen, B.O., Duus, J.Ø., and Barrero, A.F. 2002. Penicillium digitatum metabolites on synthetic media and citrus fruits. Journal of agricultural and food chemistry. 50(22): p. 6361-6365.
23. Holmes, G., Eckert, J., and Pitt, J. 1994. Revised description of Penicillium ulaiense and its role as a pathogen of citrus fruits. PHYTOPATHOLOGY. 84(7): p. 719-727.
24. Shen, Y., Zhong, L., Sun, Y., Chen, J., Liu, D., and Ye, X. 2013. Influence of hot water dip on fruit quality, phenolic compounds and antioxidant capacity of Satsuma mandarin during storage. Food Science and Technology International. 19(6): p. 511-521.
25. Ramful, D., Tarnus, E., Aruoma, O.I., Bourdon, E., and Bahorun, T. 2011. Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Research International. 44: p. 2088-2099.
26. Rapisarda, P., Bianco, M.L., Pannuzzo, P., and Timpanaro, N. 2008. Effect of cold storage on vitamin C, phenolics and antioxidant activity of five orange genotypes [Citrus sinensis (L.) Osbeck]. Postharvest Biology and Technology. 49: p. 348–354.
27. Obenland, D., Collin, S., Sievert, J., Fjeld, K., Doctor, J., and Arpaia, M.L. 2008. Commercial packing and storage of navel oranges alters aroma volatiles and reduces flavor quality. Postharvest Biology and Technology. 47(2): p. 159-167.
28. Shen, Y., Yang, H., Chen, J., Liu, D., and Ye, X. 2013. Effect of waxing and wrapping on phenolic content and antioxidant activity of citrus during storage. Journal of Food Processing and Preservation. 37(3): p. 222-231.
29. Cardeñosa, V., Barros, L., Barreira, J.C., Arenas, F., Moreno-Rojas, J.M., and Ferreira, I.C. 2015. Different Citrus rootstocks present high dissimilarities in their antioxidant activity and vitamins content according to the ripening stage. Journal of plant physiology. 174: p. 124-130.
30. Nagy, S. 1980. Vitamin C contents of citrus fruit and their products: a review. Journal of agricultural and food chemistry. 28(1): p. 8-18.
31. Escobedo-Avellaneda, Z., Gutiérrez-Uribe, J., Valdez-Fragoso, A., Torres, J.A., and Welti-Chanes, J. 2014. Phytochemicals and antioxidant activity of juice, flavedo, albedo and comminuted orange. Journal of Functional Foods. 6: p. 470-481.