falahati M, ariaii P, Khoshkhoo Z, Asadi G, Hoseini E. Investigation of antimicrobial, antioxidant, physical, and mechanical properties of a nano-composite film (nano-chitosan/ aloe vera) along with hydrolyzed tomato seed protein. FSCT 2025; 21 (157) :220-238
URL:
http://fsct.modares.ac.ir/article-7-76105-en.html
1- Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , p.aryaye@yahoo.com
3- Department of Food Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran
4- Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract: (98 Views)
This research aimed to investigate the physical, mechanical, antioxidant, and antimicrobial properties of a smart nanocomposite film based on chitosan/ aloe vera containing hydrolyzed tomato seed protein. For this purpose, the hydrolyzed tomato seed protein was first prepared using the Alcalase enzyme under different time conditions (30, 60, 90, 120 minutes). Then, 5 edible films including nano-chitosan, nano-chitosan and aloe vera gel with different concentrations of hydrolyzed protein (0, 0.5, 1 and 1.5%) were prepared, and the film properties were evaluated. Based on the results of the hydrolyzed protein, the hydrolyzed protein had a high protein content and degree of hydrolysis. This protein also had a high content of hydrophobic amino acids (31.78%) and aromatic amino acids (11.74%). The mechanical test results of the films showed that increasing the protein concentration led to a decrease in tensile strength and an increase in elongation at break of the polylactic acid films. According to the physical test results, increasing the protein concentration did not have a significant effect on moisture and solubility, but increased water vapor permeability and turbidity (p<0.05). the hydrolyzed tomato seed protein had high DPPH radical scavenging activities and increasing the concentration had a positive effect on these parameters (p<0.05). These films also had high antimicrobial activity against pathogenic bacteria, with higher antimicrobial activity against Staphylococcus aureus than Escherichia coli. The nanocomposite film containing 1.5% hydrolyzed protein had the highest antioxidant and antimicrobial activity (p<0.05). This study showed that hydrolyzed protein can improve the physical and mechanical properties of chitosan/aloe vera based films. Specifically, films containing 1.5% hydrolyzed protein had better properties such as higher antioxidant activity and antimicrobial activity.
Article Type:
Original Research |
Subject:
Packing and all types of coatings in the food industry Received: 2024/07/15 | Accepted: 2024/10/6 | Published: 2025/02/19