Volume 21, Issue 154 (2024)                   FSCT 2024, 21(154): 184-201 | Back to browse issues page

‎ 10.22034/FSCT.21.154.184

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Alizadeh behbahani B, Mehrnia M A, Jooyandeh H, Matori F. Isolation and identification of Limosilactobacillus fermentum ARD2 strain from local yogurt and evaluation of its probiotic, antimicrobial and safety properties. FSCT 2024; 21 (154) :184-201
URL: http://fsct.modares.ac.ir/article-7-75187-en.html
Isolation and identification of Limosilactobacillus fermentum ARD2 strain from local yogurt and evaluation of its probiotic, antimicrobial and safety properties
Behrooz Alizadeh behbahani 1, Mohammad Amin Mehrnia2 , Hossein Jooyandeh , Fatemeh Matori3
1- 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. , behrooz66behbahani@gmail.com
2- 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3- PhD. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Abstract:   (415 Views)
The crucial role of food in life has prompted a shift towards products that not only offer good quality and nutritional properties but also ensure the overall health of consumers. Probiotics, which are non-pathogenic microorganisms, can restore intestinal microbial balance when consumed in sufficient quantities. In this study, Limosilactobacillus fermentum ARD2 isolated from local yogurt was investigated for its probiotic properties. Various aspects of this strain were evaluated, including acid resistance (at pH levels of 2, 3 and 4), resistance to bile salts (at concentrations of 0.3, 0.5 and 0.7 %), antimicrobial activity using disk diffusion agar and well diffusion agar methods, resistance to common therapeutic antibiotics, evaluation of antioxidant activity, cell surface hydrophobicity, DNase enzyme activity, hemolytic activity, and biogenic amine and cholesterol absorption. The results of the acid resistance tests indicated that the viability of L. fermentum ARD2 increased as pH levels rose from 2 to 4, with a decrease observed over time at a constant pH over 3 hours. Growth was inhibited with increasing concentrations of bile salts. Antimicrobial testing revealed that the acidic and neutralized cell-free supernatant (aCFS and nCFS) had no significant antimicrobial effect on Escherichia coli, while nCFS showed no antimicrobial effect on Shigella dysenteriae. L. fermentum ARD2 exhibited resistance to Ciprofloxacin and was semi-sensitive to Penicillin and Nitrofurazone. The L. fermentum ARD2 showed considerable antioxidant activity, with DPPH and ABTS free radical inhibition rates of 41.40% and 43.60%, respectively. Additionally, it demonstrated the ability to reduce cholesterol absorption by 38.60%. The strain tested negative for DNase and hemolytic activities, and biogenic amine production was not observed. Based on these findings, L. fermentum ARD2 exhibits promising probiotic characteristics and could be utilized as a probiotic bacterium in food products.
 
Keywords: Antioxidant activity, Hemolytic activity, Common therapeutic antibiotics, Cell-free supernatant.
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Article Type: Original Research | Subject: Food Microbiology
Received: 2024/05/18 | Accepted: 2024/07/7 | Published: 2024/12/21
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