Volume 21, Issue 147 (2024)                   FSCT 2024, 21(147): 187-205 | Back to browse issues page

‎ 10.22034/FSCT.21.147.187

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Farzanfar F, Sadeghi Mahoonak A, Ghorbani M, Hosseini Qaboos S H, kaveh S. The effect of ultrasound pretreatment on the antioxidant properties of hydrolyzed protein from flaxseed meal using alcalase and pancreatin enzymes by response surface methodology. FSCT 2024; 21 (147) :187-205
URL: http://fsct.modares.ac.ir/article-7-72812-en.html
The effect of ultrasound pretreatment on the antioxidant properties of hydrolyzed protein from flaxseed meal using alcalase and pancreatin enzymes by response surface methodology
Faezeh Farzanfar1 , Alireza Sadeghi Mahoonak 2, Mohammad Ghorbani3 , Seyed Hossein Hosseini Qaboos4 , Shima Kaveh5
1- M.SC student in food chemistry, Department of Food Science and Industry, Faculty of Food Industry, Gorgan University of Agricultural Sciences and Natural Resources
2- Department of Food Science and Industry, Faculty of Food Industry, Gorgan University of Agricultural Sciences and Natural Resources , Sadeghiaz@yahoo.com
3- Professor, Department of Food Science and Industry, Faculty of Food Industry, Gorgan University of Agricultural Sciences and Natural Resources
4- Assistant Professor, Department of Food Science and Industry, Islamic Azad University, Azadshahr branch
5- Ph.D. of Food Chemistry, Department of Food Science and Industry, Faculty of Food Industry, Gorgan University of Agricultural Sciences and Natural Resources
Abstract:   (822 Views)
In recent years, researchers have identified and extracted bioactive peptides with acceptable antioxidant properties from different protein sources. Flaxseed meal, which is the main by-product of the linseed oil extraction process, contains a large amount of protein, which can be obtained by enzymatic hydrolysis to peptides with antioxidant properties. In this research, the effect of hydrolysis conditions (enzyme concentration 2.7-1% and time 30-183.64 minutes), type of protease (pancreatin and alcalase) and ultrasound pretreatment on the degree of hydrolysis and antioxidant properties (DPPH free radical inhibition, Total antioxidant activity, and iron ion chelation) of hydrolyzed protein obtained from flaxseed meal were evaluated using the response surface method. The optimal conditions for the production of hydrolyzed protein with the most antioxidant properties with alcalase enzyme with and without pretreatment and pancreatin enzyme with and without pretreatment, respectively, were hydrolysis time 79, 146.79, 111.77 and 97.21 minutes and enzyme concentration 2.29%, 1.46%, 2.26% and 1.38%; According to the obtained results, by examining the degree of hydrolysis and antioxidant properties of the hydrolyzed proteins of flaxseed meal, hydrolyzed protein with pancreatin enzyme was suggested as the optimal treatment. Hydrolyzed protein with pancreatin enzyme with ultrasound pretreatment was reported to have 75.66% DPPH free radical inhibition, 70.39% iron ion chelating activity, total antioxidant activity with absorbance of 0.86 nm and 80.69% hydrolysis degree. Therefore, it can be stated that the hydrolyzed protein of flax seed meal with strong antioxidant capacity is a bioactive compound for use in food formulations and the production of beneficial products.
 
Keywords: antioxidant, bioactive peptide, ultrasound, flaxseed, enzymatic hydrolysis, response surface.
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Article Type: Original Research | Subject: Proteins and bioactive peptides
Received: 2023/12/7 | Accepted: 2024/01/22 | Published: 2024/04/20
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