Volume 22, Issue 162 (2025)
FSCT 2025, 22(162): 29-41 |
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Rabiei S M, Yeganeh S, Esmaeili Kharyeki M. Investigating the antioxidant properties of hydrolyzed protein with different molecular weights obtained from Caspian Sea mullet wastes. FSCT 2025; 22 (162) :29-41
URL: http://fsct.modares.ac.ir/article-7-72106-en.html
URL: http://fsct.modares.ac.ir/article-7-72106-en.html
1- graduated
2- Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran ,skyeganeh@gmail.com
3- Assisstant Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2- Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran ,
3- Assisstant Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
Abstract: (92 Views)
Aquatic wastes management through the production of value-added products is very important. Among these products with many properties (including foaming, emulsifying, antioxidant, antibacterial, etc.), hydrolyzed proteins can be mentioned. Various factors, including molecular weight and concentration of hydrolyzed proteins, can affect the mentioned properties. Therefore, in the present study, the antioxidant properties of hydrolyzed protein obtained from Caspian Sea mullet wastes were investigated in different ranges of molecular weight and different concentrations. For this purpose, fish waste was first hydrolyzed with alcalase (1% concentration) at 55°C and pH = 8 for 120 minutes (Optimum conditions based on pre-test). Then, the hydrolyzed protein solution was divided into molecular weights of less than 3, between 3 and 10 kDa, and more than 10 kDa with 3 and 10 kDa ultrafilters. In order to evaluate the effect of molecular weight and concentration on the antioxidant activity of hydrolyzed protein, the indices of DPPH and ABTS free radicals scavenging power and ferric ion reducing power were used. Evaluation of DPPH and ABTS radicals scavenging activities showed that there is a significant difference between different molecular weights, and the highest and lowest values were recorded in molecular weights of 3-10 and less than 3 kDa, respectively (p<0.05). The lowest IC50 values for DPPH and ABTS radicals scavenging power were also obtained for sample with molecular weight between 3 and 10 kDa (p<0.05). The free radical scavenging power and the ferric ion reduction power increased with increasing concentration in all molecular weights (p<0.05). Among the different fractions, the sample with a molecular weight of more than 10 kDa showed the highest ferric ion reducing power (p<0.05). According to the results, it can be stated that hydrolyzed protein produced in this research can be used as an antioxidant compound (especially in the molecular weight of 3-10 kDa).
Article Type: Original Research |
Subject:
Fisheries and products technology
Received: 2023/10/24 | Accepted: 2025/01/18 | Published: 2025/07/23
Received: 2023/10/24 | Accepted: 2025/01/18 | Published: 2025/07/23
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