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In this study, using thyme medicinal plant without using heat by osmotic method, sugar solution with maximum active ingredients was produced. Effects of temperature (25, 35 and 45 degrees Celsius), sucrose osmotic solution concentration (40, 50 and 60%) and sampling time (15, 30, 60, 90, 120, 180, 240 and 360 minutes) on the amount The removal of active ingredients from the plant and the change in pH values and electrical conductivity (EC) were evaluated. Then the effect of blanching with hot water in (30, 60, 120 and 180 seconds) and ultrasound (0, 15 and 30 minutes) on the osmotic processing were investigated separately. The highest EC was in the solution with 40% sucrose concentration and 45°C and the lowest was in osmotic solution with 60% concentration and 45°C. Blanching before osmosis resulted in increased pH and EC. Blanching for 30 seconds caused the greatest increase in EC. Treatment with 30 minutes ultrasound increased the pH and EC in samples compared to 15 minutes of ultrasound. The chromatographic results of the control samples and the sample containing thyme extract in sucrose solution showed the release of polar and non-polar active compounds in the osmotic solution. The release of different compounds showed a difference in their release rate during the osmosis process. Finally the results showed that the extraction of active ingredients of medicinal plants was possible by osmosis process, so that the pH and EC changed over time. The product of this research can be used in the production of beverage syrups and food. In this method, the damages of other methods of extraction of effective substances such as solvent extraction, use of heat in extraction and drying of medicinal plants such as distillation and essential oil extraction methods are minimized.

Keywords: Thyme, Extraction, Effective Compounds, Blanching, Electrical Conductivity

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