Volume 5, Issue 16 (2008)
FSCT 2008, 5(16): 75-81 |
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Production of EPA by Shewanella putrefaciens MAC1 in Selected Culture Media. FSCT 2008; 5 (16) :75-81
URL: http://fsct.modares.ac.ir/article-7-10447-en.html
URL: http://fsct.modares.ac.ir/article-7-10447-en.html
Abstract: (8342 Views)
The EPA production of a marine bacterium, Shewanella putrefaciens MAC1 in four selected culture media and at incubation temperatures of 4°C for 72h and 10°C for 24h was studied. Eicosapentaenoic acid was found to be the sole polyunsaturated fatty acid produced by this bacterium. The major saturated fatty acids produced were palmitic and myristic acids. The highest yield of EPA production was 34.7 mg/g dry cell weight which was obtained in a basic culture medium containing 1% peptone and 0.5% yeast extract in ½ strength artificial sea water (ASW) at 10°C. The least EPA was produced when 2% glucose was added to the basic ingredients, indicating that EPA production is enhanced by culture media having limited source of carbon. Addition of 2 mg/l desaturase cofactors NADPH (Nicotinamide Adenine Dinucleotide Phosphate) and FAD (Flavin Adenine Dinucleotide) to the basic ingredients had a significant effect on the EPA production (21mg/g dry cell weight) at 4°C.
Received: 2008/05/2 | Accepted: 2009/01/9 | Published: 2012/09/3
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