Activated water with cold-plasma has emerged as a non-toxic and environmentally friendly alternative, demonstrating its efficiency against a wide range of pathogens, including bacteria, viruses, and fungi. In this study, tap water was activated using cold plasma generator, employing a plasma reactor consisting of copper and steel electrons, with a voltage of 20 kV and a current of 3 mA at atmospheric air. To produce and optimization of activated water, experimental designs were conducted using Design Expert software version 12, following a Box-Behnken design. The factors were treatment time (0, 15, and 30 min), air injection velocity (0.5, 1, and 1.5 m/s), and storeage temperature (20, 4, and -20 ℃). The characteristics of water, including hydrogen peroxide concentration, oxygen levels, total hardness, ozone, nitrate, nitrite, and chlorine, were measured at the days of 0, 1, 2, 3, and 6 to investigate the activation during storage period. The results indicated that all studied factors had a significant effect on the outcomes examined. The interaction effects of these factors also exhibited significant decreasing impact on the results in certain cases. Finally, it was determined that the storage temperature of the environment, prolonged treatment time with cold-plasma, and an air velocity of 1 m/s could yield activated water with superior oxidative properties. The optimized condition for producing activated water was identified as a treatment time of 23.5 min, 0.97 m/s air velocity, and storage temperature of 20℃.
Article Type:
Original Research |
Subject:
Statistics, modeling and response levels in the food industry Received: 2024/11/14 | Accepted: 2025/03/11 | Published: 2025/06/22