Ghasemi P, Alizadeh Behbahani B, Noshad M. Evaluation of antimicrobial activity of Urtica dioica leaf aqueous extract against a number of Gram-positive and Gram-negative bacteria “in vitro”. FSCT 2024; 21 (154) :173-183
URL:
http://fsct.modares.ac.ir/article-7-74653-en.html
1- 1- Ph.D. Student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2- 2- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. , behrooz66behbahani@gmail.com
Abstract: (281 Views)
Medicinal plants have been used since ancient times as a source for treating diseases. Nettle (Urtica dioica) possesses anti-inflammatory, diuretic, antihistamine, antimicrobial, and immune-boosting properties.. The aim of this study was to investigate the antimicrobial effect of nettle leaf aqueous extract using agar disc diffusion, agar well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods against a number of Gram-positive and Gram-negative bacteria. Results from agar disc diffusion and agar well diffusion tests showed that the most sensitive and resistant fungal strains to nettle leaf aqueous extract were Staphylococcus aureus and Salmonella typhi, respectively. As the concentration of nettle leaf extract increased, the zone of inhibition for all tested fungal strains significantly increased. The results indicated that the minimum inhibitory concentration for gram-negative bacteria such as Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa was 128, 256, and 256 mg/ml, respectively, and for gram-positive bacteria including Listeria innocua, Staphylococcus aureus, and Bacillus cereus was 128, 64, and 128 mg/ml, respectively. The minimum bactericidal concentration for all tested strains was greater than 512 mg/ml. This study suggests that nettle leaf aqueous extract can be used as a natural antimicrobial compound in the food industry or in the treatment of certain infectious diseases.
Article Type:
Original Research |
Subject:
Essences and extracts Received: 2024/04/12 | Accepted: 2024/06/26 | Published: 2024/12/21