Volume 17, Issue 100 (2020)
FSCT 2020, 17(100): 57-66 |
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Hanachi P, Hosseinpour M, Bathaie S Z, Zarringhalami R. Anticancer Effect of Fumaria vaillanti extracts on BT-474 and MDA-MB_123 breast cancer cells. FSCT 2020; 17 (100) :57-66
URL: http://fsct.modares.ac.ir/article-7-39070-en.html
URL: http://fsct.modares.ac.ir/article-7-39070-en.html
1- Associate Professor, Biotechnology Dep, Faculty of Biological Science, Alzahra University, Tehran Iran , p.hanachi@alzahra.ac.ir
2- MSc, Biotechnology Dep, Faculty of Biological Science, Alzahra University, Tehran Iran
3- Professor Biochemistry Department, Modares University Tehran, Iran
2- MSc, Biotechnology Dep, Faculty of Biological Science, Alzahra University, Tehran Iran
3- Professor Biochemistry Department, Modares University Tehran, Iran
Abstract: (3040 Views)
Abstract
Free radicals cause many diseases in human. Antioxidants reduce the risk of cardiovascular disease and stroke by neutralizing free radicals and on the other hand, prevent progression of cancer. The natural antioxidant enhances antioxidant properties of plasma to prevent diseases such as heart disease, cancer, and stroke. Plants are a rich source of secondary compounds, which are the most important natural antioxidants.
In this study, Fumaria vaillanti aqueous, methanol and ethanol extracts were used to determine the anticancer effect of extracts on Breast Cancer Cells Lines BT-474, MDA-MB_231 after 24, 48 and 72h.
The highest percentage of cell death, according to the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) technique, has been reported in the aqueous extract on MDA-MB-231 cells after 72 hours at with IC50 of 2 μg / ml.
The results of this study indicate that Fumaria vaillanti shows a significant antioxidant and cell toxicity effect and full potential of extracts can be realized by further studies on animal models and subsequent trials.
Free radicals cause many diseases in human. Antioxidants reduce the risk of cardiovascular disease and stroke by neutralizing free radicals and on the other hand, prevent progression of cancer. The natural antioxidant enhances antioxidant properties of plasma to prevent diseases such as heart disease, cancer, and stroke. Plants are a rich source of secondary compounds, which are the most important natural antioxidants.
In this study, Fumaria vaillanti aqueous, methanol and ethanol extracts were used to determine the anticancer effect of extracts on Breast Cancer Cells Lines BT-474, MDA-MB_231 after 24, 48 and 72h.
The highest percentage of cell death, according to the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) technique, has been reported in the aqueous extract on MDA-MB-231 cells after 72 hours at with IC50 of 2 μg / ml.
The results of this study indicate that Fumaria vaillanti shows a significant antioxidant and cell toxicity effect and full potential of extracts can be realized by further studies on animal models and subsequent trials.
Keywords: Anticancer activities, Fumaria officinalis, MTT Test, Breast Cancer, Flow Cytometry Technique
Article Type: Original Research |
Subject:
Food quality control
Received: 2019/12/16 | Accepted: 2020/03/1 | Published: 2020/05/30
Received: 2019/12/16 | Accepted: 2020/03/1 | Published: 2020/05/30
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