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At present, demands for inexpensive and alternative protein sources for animal protein, in order to be used in value–added foods have been increased. Therefore, many researchers shifting on production of plant protein resources. So, the objective of present study was possibility measurements of cottonseed protein concentrate production for human consumption from cottonseed meal. Three different methods, n-butanol : acid, mixed solvents (water : acetone : hexan) and ethanol-ultra filtration, in order to produce cottonseed protein concentrate were examined. Variables (percentage) were nitrogen, nitrogen solubility, fat, crude fiber, total gossypol and free gossypol. Statistical analysis was performed in the three distinct statistical patterns and by using blocks completely random and examination at significantly = α 0.01. After comparing resulted data with world standard measurements, results obtained as follow: extraction methods with n-butanol : acid (9 times washing along with Hcl), using mixed solvents (30 : 67 : 3 ratio along with classic extraction) and ethanol-ultra filtration (alkaline extraction along with ultra filtration molecular weight Cut off: 20000) were suitable. The last extraction method was demonstrated as most appropriate method to produce cottonseed protein concentrate.

Keywords: Cottonseed, Cottonseed meal, Protein concentrate, Extraction methods and World standard

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