Volume 8, Issue 29 (2011)
FSCT 2011, 8(29): 29-35 |
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The use of Real-Time PCR technique for detection of transgenic potato against PVY virus compared with non-transgenic ones. FSCT 2011; 8 (29) :29-35
URL: http://fsct.modares.ac.ir/article-7-5503-en.html
URL: http://fsct.modares.ac.ir/article-7-5503-en.html
Abstract: (4765 Views)
Comparison between GM potatoes against non-GM potatoes is considered as a serious obstacle in trading and research institutes in Iran. The use of new analytical methods based on genome evaluation for differential identification between GM/non-GM products has a great importance.
Sampling has been carried out from the market of four provinces. After DNA extraction according CTAB method, normal PCR and Real Time-PCR has been used for following up the target and endogen genes, 35S promoter, cp gene and Sucrose synthase gene/B-fructasidase respectively. Absence of amplification in the aforementioned PCRs, conducted the analyses to follow up PVYO and PVYN through the specific primers designed for their detection. All samples have provided good amplification implying contamination of samples by viruses and absence of GM potatoes in Iran market, as well.
Received: 2010/05/31 | Accepted: 2010/12/31 | Published: 2011/07/1
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