Volume 18, Issue 114 (2021)                   FSCT 2021, 18(114): 319-332 | Back to browse issues page

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Naghadian Moghadam M, mahmoodi E. Investigation of protein content and the relationship between enzymatic activity and antimicrobial effect of Iranian honey samples. FSCT 2021; 18 (114) :319-332
URL: http://fsct.modares.ac.ir/article-7-47845-en.html
1- Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
2- Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran. , e.mahmoodi_kh@kashanu.ac.ir
Abstract:   (1310 Views)
Honey is a natural product that has long been used for treatment in addition to nutrition. In this regard, the antimicrobial effect of some Iranian kinds of honey against antibiotic-resistant bacterial strains and the relationship between this effect and the enzymatic activity of honey samples were evaluated. In this study, the protein content of 20 honey samples before and after extraction was determined and analyzed by SDS-PAGE technique. Then the activity of glucose oxidase, amylase, invertase, and catalase enzymes of each honey was measured. Inhibition of the tested bacterial strains in the presence of honey was investigated by ager well diffusion method and the relationship between enzymatic activity with antibacterial effect and freshness of honey was evaluated. The protein content of the samples before extraction ranged from 0.4 06 0.06 to 1.0 64 64.19 mg/g honey and after extraction this amount decreased by about 50% or less. In the protein profile of all kinds of honey after electrophoresis, 3 bands related to amylase, invertase, and glucosidase enzymes were visible. The activity of enzymes was assigned to glucose oxidase> invertase> amylase> catalase, respectively. The selected honey samples did not affect Staphylococcus epidermidis and had the least effect on Pseudomonas aeruginosa. After honey inoculation, the inhibitory pattern of Escherichia coli and Enterococcus faecalis were similar, but a different inhibitory pattern was observed from Staphylococcus aureus strain. This study showed that glucose oxidase plays an important role in bacterial inhibition, but this role fluctuates in catalase-positive strains. Also, Amylase and invertase enzymes can be a measure of the freshness of honey.
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Article Type: Original Research | Subject: Food Microbiology
Received: 2020/11/22 | Accepted: 2021/03/1 | Published: 2021/08/1

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