Volume 17, Issue 106 (2020)
FSCT 2020, 17(106): 95-107 |
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Hanachi P, Salehizadeh S, Ramezani R, Zarringhalami R. Comparison of antioxidant and anti-bacterial activities of Ocimum basilicum and Impatiens walleriana and their anticancer properties on SKOV-3 cancer cell line. FSCT 2020; 17 (106) :95-107
URL: http://fsct.modares.ac.ir/article-7-40209-en.html
URL: http://fsct.modares.ac.ir/article-7-40209-en.html
1- Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN , p.hanachi@alzahra.ac.ir
2- Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN
3- Biomedical Research group, Women’s Research Center, Alzahra University, Tehran, IRAN
2- Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN
3- Biomedical Research group, Women’s Research Center, Alzahra University, Tehran, IRAN
Abstract: (2261 Views)
Abstract
Ovarian cancer is one of the deadliest cancers in women causing abnormal cancerous cells that can spread and metastasize to other parts of the body. Secondary metabolites are highly variable in terms of structural and chemical properties and they are very important for plants survival. In this study, the extracts of two plants using three different solvents (water, ethanol 80% and methanol 80%) and water bath extraction method were obtained. phenolic compounds of extracts were determined by Folin Sioculto method. DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and FRAP (Ferric reducing ability of plasma) methods were performed for determination of antioxidant properties. The amount of phenolic compounds in two plants was higher in 80% methanol solvent and the most antioxidant activities were observed in water solvent of extracts. Water solvent had the highest antioxidant activity and was selected to investigate the anti-cancer properties of extracts on ovarian cancer cells. Water extract of Ocimum basilicum within72 hours had the highest cytotoxic effect with an IC50 value of 1.105 ± 0.001 mg / ml. In study of antimicrobial activity by disc diffusion method, the highest antibacterial properties were only in ethanol extracts of two plants. In the measurement of MIC and MBC, ethanol and methanol extracts of Ocimum basilicum and water and ethanol extracts in Imatiens walleriana had the most inhibitory and bactericide effects.
Ovarian cancer is one of the deadliest cancers in women causing abnormal cancerous cells that can spread and metastasize to other parts of the body. Secondary metabolites are highly variable in terms of structural and chemical properties and they are very important for plants survival. In this study, the extracts of two plants using three different solvents (water, ethanol 80% and methanol 80%) and water bath extraction method were obtained. phenolic compounds of extracts were determined by Folin Sioculto method. DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and FRAP (Ferric reducing ability of plasma) methods were performed for determination of antioxidant properties. The amount of phenolic compounds in two plants was higher in 80% methanol solvent and the most antioxidant activities were observed in water solvent of extracts. Water solvent had the highest antioxidant activity and was selected to investigate the anti-cancer properties of extracts on ovarian cancer cells. Water extract of Ocimum basilicum within72 hours had the highest cytotoxic effect with an IC50 value of 1.105 ± 0.001 mg / ml. In study of antimicrobial activity by disc diffusion method, the highest antibacterial properties were only in ethanol extracts of two plants. In the measurement of MIC and MBC, ethanol and methanol extracts of Ocimum basilicum and water and ethanol extracts in Imatiens walleriana had the most inhibitory and bactericide effects.
Article Type: Original Research |
Subject:
Food quality control
Received: 2020/01/27 | Accepted: 2020/08/1 | Published: 2020/11/30
Received: 2020/01/27 | Accepted: 2020/08/1 | Published: 2020/11/30
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