Volume 17, Issue 103 (2020)                   FSCT 2020, 17(103): 191-200 | Back to browse issues page

‎ 10.52547/fsct.17.103.191

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Sohrabi L, fazlara A, Pourmahdi broojeni M. Comparative Evaluation of Contamination to Coliforms and E.coli in Foods by Using Different Chromogenic Medias and Their Correlation with Reference Method. FSCT 2020; 17 (103) :191-200
URL: http://fsct.modares.ac.ir/article-7-33044-en.html
Comparative Evaluation of Contamination to Coliforms and E.coli in Foods by Using Different Chromogenic Medias and Their Correlation with Reference Method
Leila Sohrabi1 , Ali Fazlara 2, Mahdi Pourmahdi broojeni3
1- Graduated of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran , a.fazlara@scu.ac.ir
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
Abstract:   (1950 Views)
Various methods have been developed to detect the presence of contamination with Coliforms and E. coli in foods. The pour plate technique using VRBA medium is confirmed as a standard method in Iran. The biggest disadvantage of this method is the need to spend a lot of time, high volume of laboratory operations, multiple stages and ultimately long time to achieve the results. Today, due to the mass production of various foodstuffs, the need for faster methods with high sensitivity to control the quality of food is necessary for the responsible oversight centers. The use of chromogenic medias is also one of the fastest diagnostic methods that have been developed. According to this matter, a comparative study was considered from the results of application of three chromogenic media and reference method in food quality control. Totally 100 samples of foodstuffs were evaluated for contamination to Coliform and E.coli using standard method and three chromogenic medias. Based on the obtained results, 86, 79, 85 and 80 samples were contaminated to Coliform using the standard method, Coliform Agar ES, ChromAgar ECC and Rapid E.coli 2/Agar respectively. Also, using four mentioned methods, contamination to E.coli was reported at 66, 80, 84 and 80, respectively. Cochrane test showed that there was no significant difference between the four methods in Coliform diagnosis (p>0.05). However, four methods did not have the same function in the detection of E. coli so that the chromogenic methods showed significant difference with the standard method (p< 0/001). But there was no significant difference between these chromogenic methods in detecting E.coli (p>0.05). According to the statistical analysis of present study, three chromogenic medias can be used instead of the standard method for the detection of Coliform, but the use of these chromogenic medias is not recommended for the identification of E.coli. 
Keywords: Coliforms, E. coli, Chromogenic, Reference Method
Full-Text [PDF 224 kb]   (1570 Downloads)    
Article Type: Original Research | Subject: Food quality control
Received: 2019/05/17 | Accepted: 2020/02/16 | Published: 2020/08/31
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