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Copolymer   Polyhydroxyalkanoat (PHAs) is the most well known degradable product of Poly-beta-hydroxybutyrate (PHB). In this research, for producing this kind of polyesters, Azotobacter was separated from soil, and then purified. By changing culture media conditions such as saturated and unsaturated oils( palmitic  acid, Olive oil,Castor oil, Sesame oil, Sprout wheat oil ,Almond oil, Sweet almond oil,Coconut oil, Snake oil, Nigella seeds oil ,Myrtles oil ,Walnut oil ,Paraffin, Stearic acid, Oleic acid, Chamomile oil, Glycerin), nitrogen, temperature and air blowing, optimum biopolymer growth condition inside the cell was evaluated. With using quantities and qualities methods like spectrophotometer assay and percentage counted by using gas chromatography (GC), and rate of PHB production were investigated. Result shows that among 10 separated Azotobacter strains, AZ7 was capable to produce 80.793% amount of PHB. Maximum amount of this biopolymer (84.48 %) in 2 % Almond oil as carbon source, 1% yeast extract as nitrogen source, 200 rpm air blowing and 33°C were observed. It seems that AZ7 strain accumulated 80-90% of their cellular dry weight of PHB in different media and in competitive of foreign strain is a native candidate for production of PHB.

Keywords: PHB, PHB, Key words: Azotobacter, Biopolymer, Saturated and unsaturated oils, Degradable plastics

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