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Atropine and scopolamine are two important tropane alkaloids, widely use as anticholinergic drugs. Such as most solanaceae plants, Atropa belladonna produces these two tropane alkaloids. Carbohydrates are not only act as carbon sources for solanaceus plants in vitro cultures, but also have some effects as signal molecules on tropane alkaloids metabolism. Effect of sorbitol, mannitol and sucrose on growth, chlorophyll, hyoscyamine and scopolamine contents, and hyoscyamine 6- beta hydroxylase (H6H) gene expression, investigated. These treatments decreased the growth of plantlets but increased chlorophyll, hyoscyamine and scopolamine contents. The treatments increase h6h gene expression in different organs of Atropa belladonna plantlets. There was an agreement between h6h expression pattern and scopolamine contents.

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The aim of this study was to establish and optimise a simplified method of halved grain culture, in order to utilise it to evaluate the ability of isolated sinks (developing wheat grains) in sucrose uptake and conversion (defined by sink strength) under unlimited source conditions. Both sucrose uptake and starch synthesis increased linearly over the incubation periods used in this study. A biphasic pattern of sucrose uptake in response to external sucrose concentrations was evident. Starch synthesis also increased curvilinearly with respect to external sucrose concentration. 2,4-dinitrophenol (DNP)) inhibited both sucrose uptake and starch synthesis. An increase in pH from 6.5 to 8 reduced both sucrose uptake and, to a lesser extent, starch synthesis. From these observations, a metabolically active uptake of sucrose by endosperm cells concomitant with a diffusional influx of sucrose into the endosperm cells were both evident. The relative importance of both the active and diffusional movement of sucrose and the importance of transport across the plasma membrane is discussed in the text.

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The use of enzymes in organic solvents has biotechnological and industrial importance. Organic solvents can decrease the stability of enzymes that is a challenge for the use of enzymes in organic media. There are several approaches such as protein engineering, chemical modification, and use of additives for stabilization of enzymes in organic solvents. In this study, activity and stability of trypsin were investigated in the presence of different organic solvents. Then the effect of sucrose on the stability of the enzyme was investigated in the absence and prescence of solvents. The result showed that the activity and stability of trypsin were decreased in the presence of organic solvents. DMF had a lowest effect on the activity and stability of the enzyme. The use of sucrose increased the stability of trypsin in the presence of organic solvents. The stabilization effect of sucrose in the presence of DMF was more than other solvents. Consequently, a mixture of DMF and sucrose is proposed for the use of trypsin in industrial applications.

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The goal of  this research was comparing physicochemical  stability of non alcoholic carbonated beverages contained high fructose corn Syrup and sucrose during storage. Soft drinks were made with three formulations of lemon , cola and orange soft drinks and in their formulations ,sucrose was replaced with 60%, 80% and 100%  of HFCS (55%) and were stored at 4 ºC, 25 ºC and 40 ºC  for one , two and four months. Samples  were analysed for titrable acidity, pH, density,  brix and color stability. pH, brix and density decreased although  titrable acidity increased during storage. There was no significant difference between samples contained  HFCS and sucrose(P>0.05). Results showed that  color of samples were stable at different temperatures ( 4 ºC, 25 ºC and 40 ºC) of storage up to four months(P<0.05) but exposing to sun light made Carmoisine unstable and HFCS intensified this effect.

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Objective: the aim of this study was to evaluate the effect of sucrose on follicular survival rate and the incidence of apoptosis in rat ovarian tissue following vitrification. Methods: Ovaries of approximately 5-week-old female Wistar rats were divided randomly into three groups: control (non-vitrified), V_I (Ethylene Glycol + Dimethyl Sulfoxide) and V_II (EG + DMSO + 0.25 mol/lit sucrose). Vitrified-warmed samples were incubated for approximately 30 minutes and fixed in Bouin’s fixative. The samples were serially sectioned and stained either with H&E or immunohistochemistry kit of anti-active and a pro-caspase-3 kit. Results: Data analysis showed that the rate of growing follicles that survived, with the exception of primordial follicles, was comparable between the vitrified-warmed and control samples. Morphologically healthy primordial follicles showed significant reductions in all vitrification groups compared to the control, however this rate was not significant between the vitrification groups. In comparison with healthy follicles, there were significantly more dead follicles in the vitrification groups than the control group. In addition the apoptotic follicles increased significantly after vitrification, with the exception of the antral follicles. Although the number of apoptotic follicles was similar between both vitrification groups, however there were significantly more pre-antral apoptotic follicles in the V_II group compared to the V_I and control groups. Conclusion: According to these results, the presence or absence of sucrose has no significant effect on the preservation of primordial and primary follicles which are important for transplantation.

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Effect of different CO₂ concentrations on sucrose metabolizing enzymes and on carbohydrate metabolism was studied for eight blackgram (Vigna mungo L. Hepper) genotypes grown in open top chambers under ambient (380 µmol mol^-1) vs. elevated CO₂ (550 and 700 µmol mol^-1) levels. The higher acid invertase activity over neutral invertase indicated the major role of acid invertase in sucrose breakdown. Higher acid invertase activity over Sucrose Synthase (SuSy) suggested the major role of invertase in sucrose breakdown and sucrolysis. Sucrose Phosphate Synthase (SPS) activity did not match with sucrose pool sizes in mature leaves and rather varied among genotypes. Plants exposed to higher CO₂ concentrations showed higher starch and sucrose contents as compared with those exposed to ambient CO₂. Leaf starch content being found several-folds higher than sucrose throughout the study indicated its major role in regulating assimilate partitioning. Increase in glucose vs. fructose concentrations for genotypes grown under elevated CO₂ conditions ranged from 20 to 90% and from 10 to 140%, respectively. The hexoses/sucrose ratio for elevated CO₂ concentration was approximately 0.8-1.6, however for ambient CO₂ content it approximately amounted to unity. Genotypes IC436720, IC519805, IC343952, and IC282009 with low hexose/sucrose ratio representing high CO₂ assimilation along with high sucrose formation indicated better tolerance to elevated CO₂ for carbon partitioning and carbohydrate metabolism. The up-regulation of leaf carbohydrate metabolizing enzymes of low hexose/sucrose as well as low sucrose/starch ratios for the genotype IC436720 (as compared with other genotypes) improved its photosynthetic capability which coupled with its better efficiency of carbon partitioning (indicative of better acclimation to elevated CO₂) could prove beneficial to its growth and productivity in the future change of climatic conditions.

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At the present study the quality and the physicochemical properties of the honey from east Azarbaijan markets were evaluated. In this study, 15 honey samples from Ahar, Tabriz and Kaleibar were collected randomly. At first the samples were transferred to the laboratory and preparation for tests such as solids content, moisture, pH, specific gravity, acidity, reducing sugars and non-reducing sugars (sucrose) and hydroxyl-methyl-furfural were performed. The results indicated that the moisture content for all samples are lower than world standards. Honey Samples from Ahar and Tabriz regions significantly had higher content of soluble solid compounds and specific gravity in compared to Kaleibar honey samples. The samples from Tabriz and Kaleibar significantly had high acidity and lower pH in compared to Ahar samples (P <0.05). Furthermore, all samples had desirable content of reducing sugars, non-reducing sugars (sucrose) and hydroxyl-methyl-furfural according to Codex and International Standards. The results of this study showed that the honey samples that were obtained from Tabriz, Kaleibar and Ahar regions have desirable quality characteristics according to national and international standards.

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Honey is one of the most important bee products that have a high nutritional value and many beneficial drug properties. And perform a variety of fraud in honey because of concerns over quality control of the product. In the present study, the Honey Tree (A₁) sample with its counterfeit samples was compared with 10% substitution with sucrose powder (A₂), 20% replacement with sucrose powder (A₃) and 30% replacement with sucrose powder (A₄), respectively. The results of this study showed that the amount of reducing sugars before hydrolysis in the range of 77.51-62.8 g%, the reducing sugars after hydrolysis in the range of 1.61 to 22%, the ratio of fructose to glucose from 0.44 to 0.33, 1, ash in the range of 0.04%, electrical conductivity in the range of 0.21-0.20%, diastase in the range of 11.47 to 20.25 (DN), Hydroxymethylfurfural (HMF) 2. 28 to 62 mg / kg. By adding different amounts of sucrose powder, the amount of reducing sugars before hydrolysis, moisture, acidity, fructose, glucose, fructose to glucose ratio, proline, antioxidant activity, samples decreased significantly and the amount of reducing sugars after hydrolysis, Diasteric, hydroxymethylfurfural and viscosity of the samples increased significantly (P<0.05). In the present study, all the factors studied, except for the amount of pre-hydrolysis regenerative sugars, fructose-glucose ratio and hydroxymethylfurfural ratio were within the standard range and could not be useful for detecting cheating the addition of sucrose to honey.

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The date fruit is of high nutritional value and its chemical composition is unique in providing energy, minerals, and vitamins for human. Date syrup, date concentrate and liquid date sugar are among semi-finished liquid products produced from low quality dates at industrial scale. In this research, some physicochemical properties of date syrup, concentrate, and liquid sugar in comparison with sucrose solutions were studied and the possibility of replacing sucrose with date liquid products was investigated. The results showed that the main chemical component of all three date products was simple sugars of fructose and glucose. Ash and protein contents of date liquid sugar were much lower than date syrup. pH values of date liquid products were also significantly different. Concentration of total phenolic and flavonoid compounds in date syrup was much greater than date liquid sugar. Overall, processing of date syrup to date liquid sugar reduced its nutritional quality while improving technological properties. It can be concluded that in choosing date liquid products as raw materials in food formulation, for the products that brown color of date liquid products does not affect the appearance of the final products, usage of date syrup or date concentrate is proposed. However, in other foods where brownish color of date syrup or date concentrate deteriorates sensory attributes of the final products, date liquid sugar is suggested. Date liquid products are also good candidates for high sugar products with sugar crystallization problem.

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To reduce sucrose consumption in biscuits, Date Syrup (DS) and Date Liquid Sugar (DLS) were replaced with sucrose at different levels (0, 20, 40, 60, 80, and 100%) and physicochemical properties of dough and the resultant biscuits were compared. Dough pH and cohesiveness decreased while softness and adhesiveness increased with addition of DS and DLS. The pH, softness and adhesiveness of the DS dough were higher than the DLS samples. The DS and DLS biscuits had lower pH, higher ash and moisture contents than the sucrose biscuits. The DS biscuits had more ash content than the DLS sample. Addition of DS and DLS resulted in biscuits with higher density, spread ratio, harder texture, and darker color. These changes were more pronounced when DS was used. Sucrose replacement with less than 40% DS or 60% DLS resulted in biscuits with sensory characteristics similar to the control, and higher antioxidant and mineral contents.

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Ultrasound is composed of sound waves with frequency beyond the limit of human hearing. Ultrasound (sonication) can be used directly for drying or as a pretreatment before the drying process. In this study, the effect of sonication power (at three levels of 0, 75 and 150 watts), ultrasound treatment time (at three times of 10, 15 and 20 minutes) and sucrose solution concentration (at three levels of 30, 45 and 60 °Brix) on the weight reduction percentage, solid gain percentage, amount of moisture removed and rehydration percentage of banana slices treated by osmotic-ultrasound treatment were investigated. With increasing ultrasonic power, increasing in treatment time and also increasing osmotic solution concentration, the weight reduction percentage and consequently the amount of moisture removed from banana slices were increased. The weight reduction percentage of banana slices increased from 8.53% to 14.28% by increasing the ultrasonic device power from zero to 150 watts (P<0.05). With increasing sonication power, the solids gain of the samples decreased, which indicates a decrease in sucrose absorption by dehydrated samples. The solids gain percentage of banana slices decreased from 2.8% to 1.4% by increasing the ultrasonic device power from zero to 150 watts (P<0.05). With increasing osmotic treatment time, more moisture was removed from banana slices and as a result, the moisture loss percentage for these samples increased. With increasing sonication power, rehydration percentage of the samples increased, but no significant difference was observed between the samples (P<0.05).

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The increase in the need for starch in the food processing industry has led to the recognition of the effect of other food compounds on the processing of starch-based foods. Xanthan and sugar are commonly used as hydrocolloids and sweeteners, respectively, in starch-based foods. These constituents can affect the structural, thermal, adhesive (dough) and rheological properties of these types of foods. The purpose of this study is to analyze the effect of sucrose and xanthan on the physicochemical, rheological and morphological characteristics of starch pastes. In addition, the comparison of the effect of the combination of xanthan and sucrose on corn and wheat starch pastes has been done in this study. In this research, the concentration of xanthan was 1% by weight and sucrose was 5% by weight, and the mixtures were prepared based on starch (corn or wheat starch). The results showed that in the presence and absence of xanthan 1% by weight and sucrose 5% by weight, the starch dough had a significant difference in terms of solubility, swelling power, water absorption, and dough transparency (p < 0.05). Rheological tests revealed that all pastes exhibited shear thinning behavior. By adding xanthan and sucrose, the apparent viscosity, storage modulus and loss modulus of starch pastes increased. Fourier transform infrared spectra showed the change in the intensity and wavelength of absorption peaks due to the addition of xanthan and sucrose. The measurements of scanning electron microscope and X-ray diffraction proved that as a result of adding xanthan and sucrose during gelatinization, changes were made in the network structure. The results of this study can improve processing and find optimal processing conditions to produce food based on Starch like baby food, bread, sweets, etc. help.

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The intensity and application time of ultrasound treatment before the osmotic dehydration process and the use of osmotic solutions of different concentrations can result in different dehydration intensities and sucrose gain in the orange slices and also can influence important qualitative parameters. In this research, the effect of ultrasound pretreatments (three levels of ultrasound intensity and at three different times) and the sucrose solution concentration (20, 30, and 40%) on the weight loss percentage, the solid gain percentage, the amount of removed moisture and the rehydration percentage of orange slices during osmotic dehydration process was investigated. By increasing the concentration of sucrose solution from 20 to 40%, the solid gain percentage of dehydrated orange slices increased from 7.94% to 16.29% (P<0.05); But with increase in the sonication power from zero to 150 W, the value of this parameter decreased. By increasing the sucrose solution concentration from 20 to 40%, due to the increase in the osmotic pressure of the solution, the amount of weight reduction and the moisture loss percentage of orange slices increased from 5.17% to 10.58% and from 13.11% to 27.14%, respectively. With increasing the ultrasound treatment time, the amount of removed moisture from the samples increased (P<0.05). With increasing ultrasound power, the rehydration of dried samples increased from 169.9% to 186.2%, but the ultrasound time had the opposite influence, and with increasing the ultrasound treatment time, the rehydration amount of samples decreased from 181.5% to 168.9%. In general, applying 5 min of ultrasound with a power of 150 W, due to the reduction of solid gain and increase in rehydration percentage, is recommended before the osmotic dehydration process of orange slices with a solution containing 40% sucrose.
 

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The importance of functional foods, Except for their nutritional role, is due to providing physiological benefits or reducing the risk of chronic diseases. Cake is a popular product among consumer meals. Therefore, reducing the amount of sucrose in the cake by making it functional with citrus fiber and essential oils is an effective step in the grain industry. The breakfast cakes containing isomalt, orange fiber and cinnamon essential oil were prepared in 8 groups. The physicochemical, color and sensory properties of breakfast cakes were investigated. The results showed that the pH of the prepared cakes was not significantly different from the control group (p>0.05). While the variables of orange fiber, isomalt, and cinnamon essential oil were effective in maintaining the moisture content of cakes (p<0.05). As expected, the fiber content of breakfast cakes increased with the addition of orange fiber (p<0.05). The fat and protein content of the treatments was lower than the control sample. The protein of the treatments did not have a significant difference(p>0.05); however, they showed a significant difference with the control treatment(p<0.05), but their ash was significantly higher than these groups (p<0.05). A significant decrease in sucrose content was reported by replacing part of it by isomalt (p<0.05). Improvement in baking volume and weight loss of breakfast cakes containing orange fiber, isomalt, and cinnamon essence was also reported (p<0.05). Examining the color index of breakfast cakes showed an increase in the L* and b* values while the a* value decreased (p<0.05). Sensory evaluation showed an increase in the acceptability of cakes containing orange fiber, isomalt, and cinnamon essential oil compared to the control sample. Considering the total results, the breakfast cake containing 2% orange fiber, 2% isomalt, and 0.1% cinnamon essence was introduced as the best treatment in this research.
 

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Purpose: The purpose of the current research is to dry apples using a solar dryer with an absorbent cycle in two modes of direct-convective radiation and indirect-convective radiation. Two types of direct and indirect drying methods with and without moisture absorbers and drying in open air were used. The pre-prepared apple slices (flavored with cinnamon) were placed on a piece of mesh with certain dimensions, the initial weight of the samples was measured and inside the solar dryer with an absorbent cycle in two modes of direct-convective radiation and indirect-convective radiation. It was placed; And during the drying process, the rate of evaporation, texture, wrinkling, water reabsorption rate, retention rate of vitamin C (ascorbic acid) and color change and sensory characteristics at different levels of treatment (30% sucrose and without sucrose and zero, 0.5, 1 and 2% of cinnamon) and also some samples were dried by solar dryer without flavoring with cinnamon alone. Data analysis was done with the completely random factorial design statistical method and using SPSS version 26 statistical software.

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