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Olive leaf consumption due to its antioxidant properties and several applications in medicine and pharmaceutical sciences is considered from ancient time. In this research effect of different drying methods including oven drying at 60 °C and 105 °C, microwave drying at 400 W and 700 W and shadow on methanolic extraction of olive leaf were evaluated. Antioxidant properties were assayed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS, and ferric reducing ability of plasma (FRAP) and also total phenolic and flavonoid content were measured using Folin-Ciocalteu and aluminum chloride respectively. Olive leaf contains 55.17% moisture, 6.12% ash, 3.18% fat, 8.21% protein, 8.58% polysaccharide, 18.48% insoluble carbohydrate, and 27.32% total carbohydrate. Results showed that drying method affect antioxidant properties and phenolic and flavonoid extraction significantly. By increasing the concentrations of extracts, their anti-radical activity increased and the radical scavenging ability of extracts depended on their concentration. Highest antioxidant property were seen in 400 W microwave and the lowest results were in oven drying at 60 °C.
 

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These days, investigation on using acoustofluidic microchannels in separation of microparticles and cells is under consideration. Working under optimum efficiency, these microchannels should be designed and manufactured truly. In this work, a new methodology for designing and manufacturing of acoustofluidic microchannels are explained. Then, a metallic microchannel with 2-nodes of pressure wave based on this method was developed. For mass production purpose, a low cost and reliable method which is CNC micromachining is used. Also, to conduct the heat generated by the wave, this microchannel was made out of aluminum and then polishing technique is applied. Then, the performance of this microchannel in agglomerating of human blood cells and BT-20 breast cancer cells to nodal lines was experimentally studied. The results showed that the applied design and manufacturing technique are suitable. Although some tests were performed to find temperature rise of microchannel due to damping effect, it was found that true design method and also using metals with high thermal conductivity can prevent the temperature increase to the point beyond which living cells will be hurt.