Evaluation of the prevalence of Vibrio cholerae isolated from water and vegetables and the presence of virulence genes and antibiotic resistance pattern in Qom province

Kermani, Somaye; Bonyadian, Mojtaba; Shams, saeed; Moshtaghi Boroujeni, Hamdollah

doi:10.22034/FSCT.21.154.128

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Tarbiat Modares University Press

Journal of food science and technology ( Iran)

Volume 21, Issue 154 (2024) FSCT 2024, 21(154): 128-138 | Back to browse issues page

‎ 10.22034/FSCT.21.154.128

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Kermani S, Bonyadian M, Shams S, Moshtaghi Boroujeni H. Evaluation of the prevalence of Vibrio cholerae isolated from water and vegetables and the presence of virulence genes and antibiotic resistance pattern in Qom province. FSCT 2024; 21 (154) :128-138
URL: http://fsct.modares.ac.ir/article-7-74432-en.html

Evaluation of the prevalence of Vibrio cholerae isolated from water and vegetables and the presence of virulence genes and antibiotic resistance pattern in Qom province

Somaye Kermani¹

, Mojtaba Bonyadian

², Saeed Shams³

, Hamdollah Moshtaghi Boroujeni⁴

1- Health and Food Quality Control.Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
2- Department of Health and Food Quality Control, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran. , boniadian@sku.ac.ir
3- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.,
4- Department of Health and Food Quality Control, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran

Abstract: (299 Views)

Vibrio cholerae is one of the important human pathogens that is transmitted through contaminated water and food. In Qom province, due to special weather conditions, diseases caused by Vibrio cholerae are endemic. The aim of this study was the prevalence of Vibrio cholerae in water and vegetables of Qom province and the presence of two virulence genes, hlyA and toxR. During two years (2020-2021), 120 samples of agricultural water (70) and vegetables (50) in Qom province were collected. The samples were cultured on specific media. Suspicious colonies were evaluated by Gram staining and biochemical tests and the serotype Vibrio cholerae was identified by serology test. Finally, Then, the presence of virulence genes was investigated by PCR method and also the antibiotic resistance pattern by disk diffusion method was evaluated in the isolates. Vibrio cholera bacteria were isolated from 17 samples (16.14%), all of which were Non-O1. The rate of contamination of water and vegetables was 28.14% (10 cases) and 14.00% (7 cases), respectively. In molecular evaluation, the abundance of virulence genes including: toxR (88.32%), rtxA (58.82%), hlyA (47.05%), chxA (5.88%), and 100% of isolates did not have ctxA, ace and tcpA genes. The most antibiotic resistance is related to ampicillin and amoxicillin (34.29%), followed by cefuroxime (17.46%), imipenem (11.76%), and cefoxitin and trimethoprim-sulfamethoxazole (5.88%). The results of this study showed that Vibrio cholerae Non-O1 is present in water and vegetables of Qom province, and as an important source of disease for humans therefore, continuous health monitoring of water and vegetables and proper disinfection of these foods is very important.

Keywords: Vibrio cholerae Non-O1, virulence genes, antibiotic resistance, water, vegetables

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Article Type: Original Research | Subject: Food Microbiology
Received: 2024/03/24 | Accepted: 2024/06/12 | Published: 2024/12/21

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