Volume 21, Issue 155 (2024)
FSCT 2024, 21(155): 58-67 |
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Ramezani R, Minatour Sajadi H, Babashah S. The effect of curcumin-loaded exosomes on the proliferation of human ovarian cancer SKOV3 cells. FSCT 2024; 21 (155) :58-67
URL: http://fsct.modares.ac.ir/article-7-74322-en.html
URL: http://fsct.modares.ac.ir/article-7-74322-en.html
1- Department of Family Therapy, Women Research Center, Alzahra University, Tehran, Iran , re.ramezani@alzahra.ac.ir
2- Department of Biochemistry, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
3- Department of Genetics, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Biochemistry, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
3- Department of Genetics, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran
Abstract: (206 Views)
Background: Curcumine is polyphenol compound which is extracted from the rhizome of the turmeric plant. Despite the anti-tumor, antioxidant and anti-inflammantaroty properties of this pigment, due to poor solubility and instability in the aqueos environments, its application in the treatment of disease has been limited. Today researchers are trying to design an efficient nanocarrier for employing this pigment in pharmaceutical application. Exosomes are lipis nanovesicles are released into extracellular environment by all types of cells and are responsible for the transfer of biological materials beween cells, so they are considered the best candidate for this goal. In this study, we aimed to load curcumin into the exosomes to increase its solubility and stability and facilitate its delivery ito ovarian cells.
Methods: After isolating the exosome by ultracentrifugation and verifying its structure with SEM, TEM and DLS, curcumine was loaded into the exosome by three methods of incubation, sonication and freez-thaw cycles. Then, using MTT assay, the toxicity effect of curcumine on SKOV3 cells was investigated.
Results: the results showed that the amount of curcumin loading into exosome was very low (less than 10%) and it was found that sonication and freez-thaw cycles have no effect on increasing the loading curcumin into the exosomes. Based on the results of toxicity test, IC50 value of free curcumin and exosome loaded curcumin was 150 and 200 respectively. Although the loading percentage of curcumin was very low, the MTT assay results showed that the loading of curcumine into the exosome plays a significant role in its efficient delivery to SKOV3 cells.
Conclusions: It can be concluded that despite the very high stability of exosomes and saffety of these cellular nanovesicles, for better efficiency in drug delivery, more studies should be done for optimizing the methods of drug loading into exosomes.
Methods: After isolating the exosome by ultracentrifugation and verifying its structure with SEM, TEM and DLS, curcumine was loaded into the exosome by three methods of incubation, sonication and freez-thaw cycles. Then, using MTT assay, the toxicity effect of curcumine on SKOV3 cells was investigated.
Results: the results showed that the amount of curcumin loading into exosome was very low (less than 10%) and it was found that sonication and freez-thaw cycles have no effect on increasing the loading curcumin into the exosomes. Based on the results of toxicity test, IC50 value of free curcumin and exosome loaded curcumin was 150 and 200 respectively. Although the loading percentage of curcumin was very low, the MTT assay results showed that the loading of curcumine into the exosome plays a significant role in its efficient delivery to SKOV3 cells.
Conclusions: It can be concluded that despite the very high stability of exosomes and saffety of these cellular nanovesicles, for better efficiency in drug delivery, more studies should be done for optimizing the methods of drug loading into exosomes.
Article Type: Original Research |
Subject:
Nanotechnology in the food industry (nanoparticles, nanocapsulations, nanomolies, etc.)
Received: 2024/03/16 | Accepted: 2024/07/31 | Published: 2024/12/21
Received: 2024/03/16 | Accepted: 2024/07/31 | Published: 2024/12/21
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