Layer by layer self - assembly technique is one of the newest methods to produce multilayer films relying on the placement of the liquid layers on solid substance. The purpose of this studies  is to manufacture multilayer kraft  based on protein biopolymers (zein and whey isolated protein) containing of green tea phenolic compounds by utilizing layer by layer self - assembly technique and evaluation antibacterial, physical-mechanical properties and release of phenolic compounds from coated kraft at refrigerator temperature in pH of 4.5 and 7. The minimum inhibitory concentration of green tea powder demonstrates that Escherichia coli (5mg/ml) compared to Listeria monocytogenes (10mg/ml) is more sensitive. The results of physical properties of krafts coated paper showed that thickness, grammage and bulk density increased by coating process. Also, the presence of zein in outer layer caused a significant reduction in WVP and increased air resistance. Evaluation of phenolic compounds release from coated krafts demonstrated that release rate in acidic food stimulant was more than the neutral stimulants. Also, release content of kraft paper with zein as outer layers respect to whey isolated protein was higher in the first days and continued in a longer time. Log reduction value of coated Kraft with active bilayer zein against Escherichia coli and Listeria monocytogenes was 0.25 and 0.06 respectively. According to the results of this study, active zein bilayer kraft is recommended as a carrier of phenolic compounds for the packaging of food products

Keywords: Layer by layer self - assembly technique, Zein, Whey isolate protein, Kraft, Green tea extract powder

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