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Mushrooms have low stability and therefore, use of applied scientific methods to increasing their stability has more important role to increasing product development. In order to study effect of nitric oxide treatment and maintenance time on physicochemical properties of mushroom (Agaricus bisporus) . A factorial experiment using completely randomized design with two concentrations of nitric oxide (0 & 4.5 mM) & 3 replication . In first time, two concentrations : 0 & 4.5 mM SNP (sodium nitroprusside) were prepared and mushrooms were dipped for 10 minutes in the solutions of nitric oxide donor, then packed in polystyrene bags, and stored at 4 ° C for 9 days, and mushroom properties were measured such as: firmness, total phenolics, ascorbic acid rate & activities of polyphenol oxidase in the days of 3, 6 and 9. The treatment of mushroom with nitric oxide significantly maintained quality at the end of the storage , So that mushrooms that containing 4.5 mM concentration nitric oxide compared with control and showed a high level of firmness, , maintained, Total phenol , ascorbic acid and reduce on activities of polyphenol oxidase on the third day in the storage. The results indicated that, the application of nitric oxide may be used as an effective and successful strategy to increase the shelf life of mushroom (Agaricus bisporus) .

Keywords: Nitric oxide, mushroom (Agaricus bisporus), phenolic compounds, polyphenol oxidase, & Browning

     

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