انجمن علوم و صنایع غذایی ایران مجله علوم و صنایع غذایی ایران 2008-8787 22 164-ویژه نامه 2025 09 23 Effect of Moringa oleifera Leaf Extract and Turmeric Supplementation on Cellular and Humoral Immune Response and Antioxidant Enzyme Activities in Shami Goats Under Heat Stress Conditions Effect of Moringa oleifera Leaf Extract and Turmeric Supplementation on Cellular and Humoral Immune Response and Antioxidant Enzyme Activities in Shami Goats Under Heat Stress Conditions 270 283 28344 10.48311/fsct.2026.118565.83010 FA Farah Najim Al-deen Al-Nasrawi National Center of Hematology, Mustansiriyah University, Baghdad, Iraq Hiba Ahmed Mahmood University of Samarra College of Education for Pure Sciences Department of Life Sciences Ghufran Nazam Abdul-Hur Al-Karkh University of Science Journal Article 2025 12 27 Heat stress is one of the significant threats to goat production in arid and semi-arid areas, which results into oxidative stress and immunosuppression. The application of Moringa oleifera leaf extract (MOLE) and turmeric (Curcuma longa) as supplements on immune parameters and antioxidant enzyme activities of Shami (Damascus) goats under heat stress was examined. Twenty-four adult Shami goats (2-3 years, 35+-3 kg) were distributed randomly in four groups (n=6): Control (CON), MOLE-supplemented (MO, 3 percent of dry matter intake), turmeric-supplemented (TUR, 2 percent of DMI) and combined supplementation (MO+TUR). The test took 60 days on a summer day ( TemperatureHumidity Index 82-92). Antioxidant enzymes (SOD, CAT, GPx), pro-inflammatory cytokines (IL-6, TNF-a), lymphocyte subpopulations (CD4+, CD8+), immunoglobulins (IgG, IgM) and heat shock proteins (HSP70, HSP90) were analyzed using blood samples collected after every 2 weeks. MO+TUR markedly enhanced SOD (MO+TUR 93.40+-2.1 U/mL) CAT (55.03+-1.8 U/mL) and GPx (15.03+-0.5 U/mL) activities in comparison to MO+TUR. The IL-6 went down to 22.1+-1.9 pg/mL (MO+TUR) versus 45.3+-3.2 pg/mL (CON) and TNF- a to 18.4+-1.7 pg/mL (P<0.001). The ratio of CD4+/CD8+ improved 1.2+-0.1 (CON) and 1.8+-0.1 (MO+TUR). The expression rate of HSP70 has gone up 2.3 times in the groups being supplemented. IgG levels were raised to 12.3+-0.8 to 16.8+-0.9mg/mL (P<0.05). A combination of M. oleifera and turmeric supplement was effective in the reduction of heat stress by improving antioxidant defensive mechanisms, inflammatory reaction, and cellular and humoral resistance against infections in Shami goats. Heat stress is one of the significant threats to goat production in arid and semi-arid areas, which results into oxidative stress and immunosuppression. The application of Moringa oleifera leaf extract (MOLE) and turmeric (Curcuma longa) as supplements on immune parameters and antioxidant enzyme activities of Shami (Damascus) goats under heat stress was examined. Twenty-four adult Shami goats (2-3 years, 35+-3 kg) were distributed randomly in four groups (n=6): Control (CON), MOLE-supplemented (MO, 3 percent of dry matter intake), turmeric-supplemented (TUR, 2 percent of DMI) and combined supplementation (MO+TUR). The test took 60 days on a summer day ( TemperatureHumidity Index 82-92). Antioxidant enzymes (SOD, CAT, GPx), pro-inflammatory cytokines (IL-6, TNF-a), lymphocyte subpopulations (CD4+, CD8+), immunoglobulins (IgG, IgM) and heat shock proteins (HSP70, HSP90) were analyzed using blood samples collected after every 2 weeks. MO+TUR markedly enhanced SOD (MO+TUR 93.40+-2.1 U/mL) CAT (55.03+-1.8 U/mL) and GPx (15.03+-0.5 U/mL) activities in comparison to MO+TUR. The IL-6 went down to 22.1+-1.9 pg/mL (MO+TUR) versus 45.3+-3.2 pg/mL (CON) and TNF- a to 18.4+-1.7 pg/mL (P<0.001). The ratio of CD4+/CD8+ improved 1.2+-0.1 (CON) and 1.8+-0.1 (MO+TUR). The expression rate of HSP70 has gone up 2.3 times in the groups being supplemented. IgG levels were raised to 12.3+-0.8 to 16.8+-0.9mg/mL (P<0.05). A combination of M. oleifera and turmeric supplement was effective in the reduction of heat stress by improving antioxidant defensive mechanisms, inflammatory reaction, and cellular and humoral resistance against infections in Shami goats. https://fsct.modares.ac.ir/article_28344_29f05888c012d13d76804c9a504c7aca.pdf