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  Emulsan compound produced by Acinetobacter calcoaceticus RAG-1 ATCC-31012. At present, this bioemulsifier show potential applications many sectors of food industry (soft cheese and ice creams). The ability of a bacterial to grow and produce emulsan by response surface methodology under shaking incubator conditions at 30°C was studied. Crude oil and ammonium sulfate at different levels use as a carbon and nitrogen source respectively. The medium culture optimum for emulsan production was occurred 4% (v/v) carbon source, 3gr/lit ammonium sulfate and 7% (v/v) inoculum’s size. At optimum levels of these parameters, 2.9 gr/lit biomass at end of fermentation was obtained. Production of emulsan by this bacterial culture resulted in surface tension reduction to 30 mN/m. Coefficients of determination, R², of fitted regression models for biomass production and surface tension are equal to 97.13% and 95.52%, respectively.

Keywords: emulsan, bioemulsifier, Surface tension, Biomass

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