Volume 7, Issue 25 (2010)
FSCT 2010, 7(25): 51-60 |
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The influence of alginate microencapsulation on survivability of microencapsulated Lactobacillus acidophilus PTCC 1643 under simulated gastrointestinal condition. FSCT 2010; 7 (25) :51-60
URL: http://fsct.modares.ac.ir/article-7-4418-en.html
URL: http://fsct.modares.ac.ir/article-7-4418-en.html
Abstract: (4497 Views)
The probiotic, Lactobacillus acidophilus PTCC 1643 was encapsulated in calcium alginate beads with the objective of enhancing survival during exposure to the adverse conditions of the gastro-intestinal tract. The probitic was incubated in simulated gastro-intestinal conditions for 0, 30, 60, 90 and 120 min. at 37 oC. The survivability of the probiotic, L. acidophilus PTCC 1643 was expressed as the destructive value (D-value). Particle size distribution was measured using laser diffraction technique. bead appearance was observed by scanning electron microscopy (SEM). The alginate coat prevented acid-induced reduction of the probiotic in simulated gastric juice (pH 1.5, 2 h), resulting in significantly (P < 0.05) higher numbers of survivors due to retarding the permeation of the gastric fluid into the cells. After sequential incubation in simulated gastric (60 min) and intestinal juices (pH 7.25, 2 h), number of surviving cells were 6.5 log cfu ml -1 for encapsulated L. acidophilus while 2.3 log cfu ml -1 was obtained for free cells.
Received: 2008/07/31 | Accepted: 2008/12/30 | Published: 2012/06/30
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