Volume 7, Issue 25 (2010)
FSCT 2010, 7(25): 61-67 |
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Optimization of medium composition for microbial production of glutamic acid from Date fruit wastes using fractional factorial method. FSCT 2010; 7 (25) :61-67
URL: http://fsct.modares.ac.ir/article-7-2797-en.html
URL: http://fsct.modares.ac.ir/article-7-2797-en.html
Abstract: (5163 Views)
Abstract
Date wastes, one of the abundantly available carbohydrate agricultural wastes in the South and East of the Mediterranean, were served as a novel substrate for the production of glutamic acid, the precursor of monosodium glutamate. A fractional factorial design was used to investigate the effects of variables, namely biotin content, urea content and agitation speed on the response glutamic acid production. A second order polynomial model was used to predict the response. Agitation speed was found to significantly influence the glutamic acid production. The highest glutamic acid production was recorded at agitation speed ranging 240-250 rpm and urea concentration of 2.8-3 g/l. The study showed that the wastes from dates could serve as a low-cost substrate for glutamic acid production.
Keywords: Glutamic acid, Date wastes, Submerge, Agitation speed, Fractional factorial design, Biotin
Received: 2008/04/29 | Accepted: 2009/02/28 | Published: 2012/06/30
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