Volume 15, Issue 84 (2019)
FSCT 2019, 15(84): 137-151 |
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Shariat alavi M, Sadeghi Mahoonak A, Ghorbani M, Alami M, Mohammadzadeh J. Determination of Optimum Conditions for Production of Hydrolyzed Protein with Antioxidant Capability and Decrease of Nitric Oxide from Tomato Wastes by Alcalas. FSCT 2019; 15 (84) :137-151
URL: http://fsct.modares.ac.ir/article-7-14632-en.html
URL: http://fsct.modares.ac.ir/article-7-14632-en.html
Mahya Shariat alavi1 
, Alireza Sadeghi Mahoonak , Mohammad Ghorbani2 , Mehran Alami2 , Jalal Mohammadzadeh3
, Alireza Sadeghi Mahoonak , Mohammad Ghorbani2 , Mehran Alami2 , Jalal Mohammadzadeh3
1- Msc student, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources
2- Associate Professors, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources
3- Agricultural Research Center
2- Associate Professors, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources
3- Agricultural Research Center
Abstract: (5140 Views)
Tomato waste can be used as a new protein source for the production of hydrolyzed protein. Free radicals are among the most important factors in the development of cancer and genetic mutations, which have become one of the greatest threats for human health in recent centuries. In this research, hydrolysis condition of tomato seed protein (temperature, time and amount of alcalase enzyme) to achieve maximum antioxidant activity and nitric oxide reducing power were investigated. The values were evaluated using Design Expert software and analyzed by the response surface methodology. Results showed that with the optimimizing hydrolysis conditions using the alcalase enzyme, it is possible to achieve a products with significant DPPH radical scavenging activity and nitric oxide reducing power. The results indicate that optimum conditions for achieving the maximum DPPH free radical inhibition activity and nitric oxide reducing power is temperature of 50 ° C, hydrolysis time 210 min, and the amount of enzyme 1.85%. Under these condition the amount of DPPH free radical inhibition activity and nitric oxide reducing power was 85.53 and 61.17, respectively. The results showed that the hydrolyzed protein produced from tomato seed could be used as a natural additive in the foods formulation and pharmaceutical uses with high antioxidants and nitric oxide reducing activity.
Keywords: Alcalase enzyme, protein hydrolysate, Response Surface Methodology, Antioxidant activity, nitric oxide inhibitory
Article Type: Original paper |
Subject:
Food quality control
Received: 2018/02/19 | Accepted: 2019/02/15 | Published: 2019/02/15
Received: 2018/02/19 | Accepted: 2019/02/15 | Published: 2019/02/15
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