Volume 18, Issue 120 (2021)
FSCT 2021, 18(120): 199-213 |
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Mardani Ghahfarokhi V, Khosrowshahi Asl A, Zomorodi S. Co-encapsulation of Lactobacillus acidophilus (La-5) and omega-3 rich oil through complex coacervation. FSCT 2021; 18 (120) :199-213
URL: http://fsct.modares.ac.ir/article-7-54219-en.html
URL: http://fsct.modares.ac.ir/article-7-54219-en.html
1- Department of Food Science and Technology, Urmia University, Urmia, Iran
2- Department of Engineering Research, West Azerbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia, Iran , shahinzomorodi@gmail.com
2- Department of Engineering Research, West Azerbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia, Iran , shahinzomorodi@gmail.com
Abstract: (1465 Views)
Co-encapsulation of Lactobacillus acidophilus and Tuna oil rich in omega-3 fatty acids using Soy protein isolate (SPI) and Arabic gum (GA) as wall materials was studied through the complex coacervation method. The microcapsules were dried separately by freeze and spray drying. The optimal conditions for the coacervation between soybean protein isolate and gum Arabic as functions of pH, SPI/GA ratio and total concentration of biopolymers were investigated using zeta potential, turbidity, and coacervation yield assays. The highest coacervate yield was achieved in the total concentration of biopolymers 2% (w/v), SPI/GA ratio 60:40 and pH=4, and the highest coacervation yield was 79.22±1.75%. Lactobacillus acidophilus (La-5) viability was significantly (p˂0.05) higher in freeze-dried microcapsules in comparison with spray-dried microcapsules. Omega-3 fatty oil improved significantly (p˂0.05) the viability of probiotic bacteria during 60 days storage in ambient temperature and gastrointestinal conditions. The size of L. acidophilius containing microcapsules and co-microcapsules (SPI-P-O-GA) were 1.19±0.19 and 4.42±0.14 µm, respectively.
Keywords: microencapsulation, Lactobacillus acidophilus, Omega-3 rich oil, Arabic gum, Soy protein isolate
Article Type: Original Research |
Subject:
Food Microbiology
Received: 2021/07/20 | Accepted: 2021/09/21 | Published: 2021/12/1
Received: 2021/07/20 | Accepted: 2021/09/21 | Published: 2021/12/1
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