Mozafari M, EMTYAZJOO M, Anvar A A. Anti-diabetic effect of probiotic milk containing Lactobacillus rhamnosus IBRC-M10754 in animal model of wistar rat and survive it in cold storage conditions. FSCT 2023; 20 (138) :16-25
URL:
http://fsct.modares.ac.ir/article-7-52740-en.html
1- Department of Food Science and Technology, Science and Research Branch, Tehran- Iran
2- Azad Islamic university North Tehran Branch , moz_emtyazjoo@yahoo.com
3- Marine science and technology ,Islamic Azad university north Tehran branch
Abstract: (1004 Views)
The aim of the present study was to evaluate the viability of Lactobacillus rhamnosus IBRC-M 10754 in milk as a probiotic in cold storage and to evaluate its anti-diabetic effects. After bacterial culture in MRS medium and addition to milk, treatments containing 107, 108 and 109 cfu / ml of probiotic milk were prepared. Bacterial viability was performed for 5 days by sequential dilution and extensive culture in three replicates. Then, gavage containing probiotic-containing milk to Balb/C male rats that were infected with Streptozotocin was compared with anti-diabetic rats fed L. rhamnosus milk by measuring their blood glucose and weight. The results showed that the highest number of live bacteria in milk at 4Cº ,belonged to the first day of storage and there was no significant difference from the second day until the end of storage period. Overall, the bacterial population in milk decreased significantly (p≤0.05) over time. Addition of 109 cfu/ml primary inoculum showed that at the end of the fifth day the bacterial population in the milk was as high as that in the probiotic milk by the end of the cold storage. Mice weighing showed that the weight of control rats increased during the storage period, but diabetic rats decreased during this time. Milk with Lactobacillus inoculated in diabetic rats at different doses of inoculation resulted in a significant (P <0.05) decrease in blood glucose during their 21-day storage period. The decline of the fourteenth day of feeding probiotics is more evident.
Article Type:
Original Research |
Subject:
Bioactive compounds Received: 2021/05/21 | Accepted: 2023/04/12 | Published: 2023/08/1