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The aim of this study was to investigate some physicochemical properties and antioxidant activity of biodegradable film prepared from sodium caseinate containing titanium nanoxide and grape seed essential oil and its effect on the oxidative resistance of olive oil. Antioxidants are added to oil to delay oxidation. According to previous studies, the carcinogenicity of traditional antioxidants has led to a reduction in their use and their replacement with natural antioxidants. For this purpose, bionanocomposite films based on sodium caseinate at different levels of titanium nanoxide (zero, 0.38, 0.75, 1.3 and 1.5% w/w) and grape seed essential oil at five levels (zero, 125, 250, 375 and 500 ppm) were prepared. The results showed that the addition of titanium nanoxide and grape seed essential oil increased the thickness and opacity of the films and reduced the light transmission through the films and thus their opacity. The film containing 1.5% of titanium nanoxide and 500 ppm of essential oil had the highest turbidity and thickness among other samples. With increasing the concentration of grape seed essential oil, the antioxidant properties of the films also increased, so that the film containing 500 ppm of essential oil showed 78% antioxidant activity.

Keywords: Sodium caseinate, Titanium nanoxide, Grape seed essential oil, Olive oil

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