Volume 17, Issue 109 (2021)                   FSCT 2021, 17(109): 33-42 | Back to browse issues page

‎ 10.52547/fsct.17.109.33

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Noshad M, Alizadeh behbahani B. The antibacterial effect of Stachys byzantina extract on a number of pathogenic bacteria using agar dilution and Cylinder-plate methods. FSCT 2021; 17 (109) :33-42
URL: http://fsct.modares.ac.ir/article-7-43801-en.html
The antibacterial effect of Stachys byzantina extract on a number of pathogenic bacteria using agar dilution and Cylinder-plate methods
Mohammad Noshad1 , Behrooz Alizadeh behbahani 2
1- Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2- Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran , behrooz66behbahani@gmail.com
Abstract:   (2847 Views)
Stachys byzantina belongs to Lamiaceae family. Regarding to the biologically active compounds and traditional use of the Stachys byzantina for treatment of wounds and a cold, it seems that this plant has significant antibacterial effects. The aim of this study was evaluation of antibacterial effect of ethanolic extract of Stachys byzantina against Enterobacter aerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus. The inhibition zone diameter of the Stachys byzantina extract on Enterobacter aerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus was identified on a concentration of 25, 50, and 75 mg/mL, respectively, by Cylinder-plate and Kirby-Bauer methods. Moreover, minimum inhibitory concentration (MIC) (agar dilution and micro-well dilution) and minimum bactericidal concentration (MBC) of the pathogenic microorganisms were determined in this study. The data were analyzed using one-way ANOVA and Duncan's multiple range test. The highest and lowest inhibition zone with a diameter of 17.90 and 13.10 mm at a concentration of 75 mg/mL were observed for Staphylococcus aureus and Enterobacter aerogenes bacteria, respectively (Cylinder-plate method). The MIC of Stachys byzantina extract for Enterobacter aerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus was 100, 50, 25 and 12.5 mg/mL respectively (agar dilution). The ethanolic extract of Stachys byzantina “in vitro” has a significant antibacterial effect on Enterobacter aerogenes and Escherichia coli (Gram-negative bacteria) and Bacillus cereus and Staphylococcus aureus (Gram-positive bacteria).
Keywords: Stachys byzantina, Cylinder-plate method, agar dilution, Kirby-Bauer method.
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Article Type: Original Research | Subject: food industry engineering
Received: 2020/06/19 | Accepted: 2020/08/22 | Published: 2021/03/9
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