Volume 13, Issue 57 (0)
FSCT 0, 13(57): 133-143 |
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Karazhiyan R, Habibi Najafi M B, Yavar manesh M, Edalatian M R, Poriyan Far H R. Evaluation suitable method for DNA extraction Aspergillus niger from tomato paste. FSCT 0; 13 (57) :133-143
URL: http://fsct.modares.ac.ir/article-7-2596-en.html
URL: http://fsct.modares.ac.ir/article-7-2596-en.html
Reza Karazhiyan 
, Mohammad Bagher Habibi Najafi , Masuood Yavar manesh , Mohammad reza Edalatian , Hamid Reza Poriyan Far
, Mohammad Bagher Habibi Najafi , Masuood Yavar manesh , Mohammad reza Edalatian , Hamid Reza Poriyan Far
Abstract: (4907 Views)
In this research different methods of DNA extraction from Aspergillus niger in tomato paste have been optimized and compared with each other. For this purpose classical optimization techniques and commercial Kit base methods were used. Mold spores of Aspergillus niger at different levels (101, 103 and 105 CFU/g) were added to the tomato paste. After mycelium growth DNA extraction was perfomed by five methods. Different methods employed by use the Liquid nitrogen, ultrasonic and lysis solution in the cell wall. These methods in terms of quality and quantity of DNA extracted were studied using spectrophotometer with NanoDrop. The absence of smear, protein contamination and no presecnce of PCR inhibitors determined with PCR that performed forward and reverse primers. Result of gel electrophoresis and PCR assay showed that Method 1 is the most appropriate method for DNA extraction is the mold of tomato paste.
Received: 2015/11/8 | Accepted: 2015/11/8 | Published: 2016/11/21
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